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. 2010 Oct 19;9:77. doi: 10.1186/1475-2859-9-77

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Copyright ©2010 Fong and Wood; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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ELP-intein-mediated purification of ELP-intein-tagged (A) β-gal and (B) OPH-S5, produced in Rosetta^TM(DE3) fed batch fermentation. Non-chromatographic purification of target proteins based on the selective precipitation of the ELP tag, using two rounds of inverse transition cycling. Lane 1: clarified cell lysate; Lane 2: soluble contaminants removed after selective ELP precipitation; Lane 3: purified EI-X precursor, before intein cleavage; Lane 4: purified EI-X precursor, after intein cleavage; Lane 5: purified target protein.