Table 1.
Product yields and cell growth in various strains and media recipes
| Shake Flask | Strain | Medium | Yield1 β-gal (mg/L) | Final OD600 |
|---|---|---|---|---|
| Rosetta | TB* | 106 | 12.8 | |
| Rosetta | NBS | 121.5 | 6 | |
| Rosetta | NBS (no IPTG) | 7.8 | 16.1 | |
| BLR | TB* | 109 | 9.7 | |
| BLR | NBS | 57 | 3.23 | |
| ER2566 | NBS | 80 | 14.6 | |
| Batch fermentations | Strain | Medium | Yield1 β-gal (mg/L) | Final OD600 |
| Rosetta | TB* (pH 6.9, dO2 > 20%) | 31 | 25 | |
| Rosetta | NBS (pH 6.9, dO2 > 20%) | 39 | 28 | |
| Rosetta | NBS (pH 6.9, dO2 < 10%) | 104 | 26 | |
| Fed-batch fermentations | Strain | Medium | Yield1 β-gal (mg/L) | Final OD600 |
| Rosetta | Feed 1 | 180 | 73 | |
| Rosetta | Feed 2 | 173 | 128 | |
| Rosetta | Feed 3 | 127 | 95 | |
| Rosetta | Feed 4 | 160 | 121 | |
| Rosetta | Feed 5 | 234 | 151 | |
| Rosetta | Feed 5 | 2362 | 129 | |
1 These yields are based on one round of inverse transition cycling to show overall yield trends. More accurate absolute yield information can be found in Table 2.
2Yield of OPH-S5 (mg/L)