Table 2.
Small-scale purification of β-gal and OPH-S5 based on two rounds of inverse transition cycling.
| A. β-galactosidase | |||
|---|---|---|---|
| Step | Specific activity (U/mg) | Purification fold | Yield (mg/L) |
| Clarified lysate | 5096 | 1 | |
| Soluble contaminants | 466 | ||
| Purified precursor, before cleaving | 76656 | 15 | |
| Purified product | 135017 | 25.5 | 177 |
| B. OPH-S5 | |||
| Step | Specific activity (U/mg) | Purification fold | Yield (mg/L) |
| Clarified lysate | 11 | 1 | |
| Soluble contaminants | 1.2 | ||
| Purified precursor, before cleaving | 751 | 68 | |
| Purified product | 1186 | 108 | 83 |