Table 3.
Component | NBS1 | TB*1 | Feed 11 | Feed 21 | Feed 31 | Feed 41 | Feed 51 | NBS fed batch (Feed 5)1 | NBS (bulk)2 | TB* (bulk) | Feed 5 (bulk) | NBS fed batch (Feed 5, bulk) | NBS fed batch (Feed 5, commodity)4 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Ammonium phosphate | 2.12 | 1.41 | 0.46 | 0.31 | |||||||||
Ammonium chloride | 0.31 | 1.25 | 2.5 | 1.25 | 0.42 | 1.09 | 0.36 | ||||||
Ammonium sulfate | 1.47 | 6.81 | 1.47 | 0.49 | 1.28 | 0.43 | |||||||
Boric acid | 0.006 | 0.004 | 0.006 | 0.004 | |||||||||
Corn steep liquor | .001 | ||||||||||||
Corn syrup | 0.07 | ||||||||||||
Cupric sulfate | 0.0006 | 0.0004 | 0.0006 | 0.0004 | |||||||||
Ferric chloride | 0.003 | 0.002 | 0.003 | 0.002 | |||||||||
Glucose | 3.65 | 94.89 | 94.89 | 94.89 | 94.89 | 94.89 | 34.03 | 0.7 | 18.2 | 6.53 | |||
Glycerol | 0.76 | 0.14 | |||||||||||
Magnesium sulfate | 0.59 | 0.89 | 0.89 | 0.89 | 0.89 | 0.89 | 0.69 | 0.02 | 0.35 | 0.13 | 0.13 | ||
Manganese chloride | 0.008 | 0.005 | 0.008 | 0.005 | |||||||||
Monosodium glutamate | 17.32 | ||||||||||||
Potassium phosphate dibasic | 0.5 | 2.07 | 0.33 | 0.18 | 0.76 | 0.12 | 0.12 | ||||||
Potassium phosphate monobasic | 0.22 | 0.25 | 0.15 | 0.15 | 0.18 | 0.10 | 0.10 | ||||||
Proline | 6.91 | 6.913 | |||||||||||
Sodium chloride | 0.002 | 0.001 | 0.002 | 0.001 | |||||||||
Sodium molybdate | 0.002 | 0.001 | 0.002 | 0.001 | |||||||||
Sulfuric acid | 0.002 | 0.001 | 0.002 | 0.001 | |||||||||
Thiamine | 0.0003 | 0.0002 | 0.0003 | 0.0002 | |||||||||
Tryptone | 1.85 | 1.853 | |||||||||||
Yeast extract | 0.96 | 4.63 | 0.96 | 0.96 | 0.963 | 4.633 | 0.963 | 0.96 | |||||
Zinc sulfate | 0.0006 | 0.0004 | 0.0006 | 0.0004 | |||||||||
Cost per L (US Dollars) | 8.06 | 16.47 | 96.09 | 98.50 | 113.10 | 105.09 | 99.46 | 38.50 | 2.49 | 14.47 | 21.88 | 8.95 | 0.42 |
1Prices based on 500 g reagent grade chemicals from Fisher Scientific.
2Bulk scale prices based on 50 kg reagent grade chemicals from Fisher Scientific, when available. Otherwise, price based on largest reagent grade size available (1-10 kg). Bulk scale pricing was not taken into account for trace materials.
3Bulk discounts not available.
4Commodity scale replaces (mass for mass) glucose with corn syrup, nitrogen sources with corn steep liquor, and eliminates trace metals.