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. 2010 Oct 19;9:77. doi: 10.1186/1475-2859-9-77

Table 3.

Media pricing

Component NBS1 TB*1 Feed 11 Feed 21 Feed 31 Feed 41 Feed 51 NBS fed batch (Feed 5)1 NBS (bulk)2 TB* (bulk) Feed 5 (bulk) NBS fed batch (Feed 5, bulk) NBS fed batch (Feed 5, commodity)4
Ammonium phosphate 2.12 1.41 0.46 0.31

Ammonium chloride 0.31 1.25 2.5 1.25 0.42 1.09 0.36

Ammonium sulfate 1.47 6.81 1.47 0.49 1.28 0.43

Boric acid 0.006 0.004 0.006 0.004

Corn steep liquor .001

Corn syrup 0.07

Cupric sulfate 0.0006 0.0004 0.0006 0.0004

Ferric chloride 0.003 0.002 0.003 0.002

Glucose 3.65 94.89 94.89 94.89 94.89 94.89 34.03 0.7 18.2 6.53

Glycerol 0.76 0.14

Magnesium sulfate 0.59 0.89 0.89 0.89 0.89 0.89 0.69 0.02 0.35 0.13 0.13

Manganese chloride 0.008 0.005 0.008 0.005

Monosodium glutamate 17.32

Potassium phosphate dibasic 0.5 2.07 0.33 0.18 0.76 0.12 0.12

Potassium phosphate monobasic 0.22 0.25 0.15 0.15 0.18 0.10 0.10

Proline 6.91 6.913

Sodium chloride 0.002 0.001 0.002 0.001

Sodium molybdate 0.002 0.001 0.002 0.001

Sulfuric acid 0.002 0.001 0.002 0.001

Thiamine 0.0003 0.0002 0.0003 0.0002

Tryptone 1.85 1.853

Yeast extract 0.96 4.63 0.96 0.96 0.963 4.633 0.963 0.96

Zinc sulfate 0.0006 0.0004 0.0006 0.0004

Cost per L (US Dollars) 8.06 16.47 96.09 98.50 113.10 105.09 99.46 38.50 2.49 14.47 21.88 8.95 0.42

1Prices based on 500 g reagent grade chemicals from Fisher Scientific.

2Bulk scale prices based on 50 kg reagent grade chemicals from Fisher Scientific, when available. Otherwise, price based on largest reagent grade size available (1-10 kg). Bulk scale pricing was not taken into account for trace materials.

3Bulk discounts not available.

4Commodity scale replaces (mass for mass) glucose with corn syrup, nitrogen sources with corn steep liquor, and eliminates trace metals.