Table 4.
Economic comparison of expression conditions
| Medium | Scale | Medium cost (per L) | Yield (mg/L) | Final OD600 | Media cost per mg protein | Time1 |
|---|---|---|---|---|---|---|
| NBS/Feed 5 (Lab scale) |
2.25 L fed batch2 fermentation | $38.52 | 2363 | 150 | 16.3 ¢ | 43-46 hours |
| NBS/Feed 5 (Bulk scale) |
2.25 L fed batch2 fermentation | $12.96 | 2363 | 150 | 5.5 ¢ | 43-46 hours |
| TB* (Lab scale) |
1.5 L batch fermentation | $16.48 | 34.54 | 25 | 47.8 ¢ | 8-12 hours |
| TB* (Bulk scale) |
1.5 L batch fermentation | $14.47 | 34.54 | 25 | 41.9 ¢ | 8-12 hours |
| TB* (Lab scale) |
50 mL shake flask | $16.48 | 1094 | 12.8 | 6.6 ¢ | 24-28 hours |
1Time reported is total time from inoculation to harvest.
2Fed batch fermentations consisted of a 1.5 L batch phase in NBS media, followed by 750 mL Feed 5 added during the fed batch and induction phases.
3Target protein was OPH-S5
4Target protein was β-gal