Table 1.
Primer pairs used in the real-time PCR experiments of gene expression profiles Primer pairs applied for real-time PCR
| Gene | Accession | Sense primer | Antisense primer | Product |
|---|---|---|---|---|
| bTERT | NM001046242 | TCACGGGTCAAGACGCTGT | CGGGCATAACTGGAGTGGT | 886 bp |
| p16INK4a | XM868375 | GGTGATGATGATGGGCAGCG | ACCAGCGTGTCCTGGAAGC | 134 bp |
| p53 | NM174201 | CGGAACACCTTTAGACACAGT | GTAGGCAGTGCTCGCTTAGT | 304 bp |
| p21Cip1 | NM001098958 | TCTACCACTCCAAACGCA | GCAAAGGATGAAGAGGGTT | 331 bp |
| TRPS-1 | NM014112* | GCCTTCTTTGAGTTCGGA | CCCTCTGCTGTTTGTTGAG | 384 bp |
| Bcl-2 | XM586976 | GCCTTCTTTGAGTTCGGA | TTCAGAGACAGCCAGGAGA | 209 bp |
| Bax | NM173894 | AGTGGCGGCTGAAATGTT | TTCTTCCAGATGGTGAGCG | 287 bp |
| Bag-1 | NM001076291 | TGCCATTGTCAGCACTTG | TGTTCTGTTCCACTGTGTCAC | 376 bp |
| c-Myc | AF519455 | GTGTCTACCCATCAGCACAA | AACTGTTCTCGCCTCTTCTG | 376 bp |
| DNMT1 | NM182651 | CCCGAGTGTGGAAAGTGTA | GAACATCTGCCCATTGCT | 392 bp |
*TRPS-1 primer pairs were designed by comparing homologous sequences between the Homo sapiens and Bos taurus genome, because the cattle TRPS-1 sequence has not been reported.
All other primer sequences were cited from GenBank. Each pairs of primers span intron-extron boundaries.