Figure 1.

DHPCC-9 inhibits Pim-1-dependent survival of cytokine-deprived myeloid cells. (A) Schematic structure of 1,10-dihydropyrrolo[2,3-a]carbazole-3-carbaldehyde (DHPCC-9). (B) FDCP1 cell lines stably expressing neomycin (FD/Neo) or Pim-1 (FD/Pim44) were cultured for 24 h with or without IL-3 in the presence of DMSO or 10 μM DHPCC-9, after which cell viability was analysed by the MTT assay. Graph represents means from three independent experiments with duplicate samples. (C) Cells were cultured for 24 h without IL-3 in the presence of increasing concentrations of DHPCC-9. Cell viability was analysed by the MTT assay and IC₅₀ values were determined. Points represent means from four independent experiments with duplicate samples. (D) Cells grown in the absence of IL-3 were stained with Trypan blue and live cells were counted at the indicated time-points. Points represent means from triplicate determinations from one of two similar experiments.