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. 2010 Oct 19;9:279. doi: 10.1186/1476-4598-9-279

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Copyright ©2010 Santio et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Enhancing effects of NFATc1 on migration of prostate cancer cells are abolished by DHPCC-9. PC-3 cells were transiently transfected with an empty vector (Mock) or a vector expressing FLAG-tagged NFATc1. DMSO or 10 μM DHPCC-9 were added 24 h after transfection. The wound healing assays were initiated 2 h later by scratching the wounds with a 10 μl sterile pipette. (A) Shown are representative pictures from three time-points. (B) Graph represents means of two similar experiments with triplicate samples. (C) Overexpression of NFATc1 was confirmed by Western blotting with anti-FLAG antibody. GAPDH staining was used as a loading control.