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. Author manuscript; available in PMC: 2011 Oct 6.
Published in final edited form as: Neuron. 2010 Oct 6;68(1):99–112. doi: 10.1016/j.neuron.2010.09.002

Figure 5. Acetoacetate modulates vesicular glutamate release from neurons but not from astrocytes.

Figure 5

A. Cultured hippocampal neurons were treated with the indicated concentrations of acetoacetate 20 min before KCl stimulation. For the “washout” experiment, acetoacetate-treated cells were washed with medium and incubated a further 1 h. KCl-dependent glutamate release was assessed for 20 min. Error bars represent mean ± SEM; n = 6. B. Hippocampal astrocytes were also treated with 5 mM acetoacetate. Glutamate secretion upon the addition of KCl was measured as described above. Error bars represent mean ± SEM; n = 6; asterisks indicate statistical significance, ***P<0.001.