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. Author manuscript; available in PMC: 2011 Oct 6.
Published in final edited form as: Neuron. 2010 Oct 6;68(1):99–112. doi: 10.1016/j.neuron.2010.09.002

Figure 7. Acetoacetate inhibited 4AP-evoked seizures and glutamate secretion.

Figure 7

A. Schematic diagram and typical protocol of 4AP-evoked seizures. At the times and duration indicated, 10 mM 4AP was added. Acetoacetate (10 mM) was added during the indicated period. B. 4AP-evoked seizures and the protective effect of 10 mM acetoacetate were observed for 1 h after each addition of 4AP and given a total seizure severity score (TSSS) based on behavioral analysis. Error bars represent mean ± SEM; n = 6. Asterisks indicate statistical significance (Student's t-test), *P<0.1, ** P<0.01, *** P<0.001. C. Typical HPLC chromatogram of glutamate and dopamine secreted at the indicated sampling times. * o-phospho-L-serine at 40 pmol as an internal marker. D. Dose dependence of acetoacetate on 4AP-evoked glutamate secretion.