Table 3.

Results on gene essentiality predictions by the finalized mouse model.

Gene Name	Reactions	Subgroup	Mutation lethal	Comment	Reference
EBP	3-beta-hydroxysteroid-delta(8),delta(7)-isomerase	Cholesterol Metabolism	yes	X-linked genes, homozygous males are non-viable (prenatal lethality)	Means et al.
DHCR7	7-dehydrocholesterol reductase	Cholesterol Metabolism	yes	Prenatal lethality of homozygotes	Yu et al.
DHCR24	24-dehydrocholesterol reductase	Cholesterol Metabolism	yes	Prenatal lethality of homozygotes	Mirza et al.
FDFT1	Squalene synthase	Cholesterol Metabolism	yes	Prenatal lethality of homozygotes	Tozawa et al.
HSD17B4	C-3 sterol keto reductase, Beta oxidation of long chain fatty acid, 3-hydroxyacyl-CoA dehydrogenase, hydroxysteroid (17-beta) dehydrogenase 4, peroxisomal lumped long chain fatty acid oxidation	Cholesterol Metabolism	yes	Pre/Peri/Postnathal lethality of homozygotes	Huyghe et al.
NSDHL	C-3 sterol dehydrogenase, C-4 methyl sterol oxidase	Cholesterol Metabolism	yes	X-linked gene, males and homozygous females are non-viable (prenatal lethality)	Cunningham et al
SC5DL	Lathosterol oxidase	Cholesterol Metabolism	yes	Perinatal lethality of homozygotes	Krakowiak et al.
SPTLC1	serine palmitoyltransferase, long chain base subunit 1	Sphingolipid Metabolism	yes	Embryonic lethality of homozygotes	Hojjati et al.
DHFR	dihydrofolate reductase, folate reductase	Folate Metabolism	yes	Embryonic lethality of homozygotes	Di Pietro et al.
PISD	phosphatidylserine decarboxylase, mitochondrial	Glycerophospholipid Metabolism	yes	Embryonic lethality of homozygotes	Steenbergen et al.
PHGDH	phosphoglycerate dehydrogenase	Glycine, Serine, and Threonine Metabolism	yes	Embryonic lethality of homozygotes	Yoshida et al.
HMGCR	Hydroxymethylglutaryl CoA reductase (ir)	Cholesterol Metabolism	yes	Embryonic lethality of homozygotes	Tanaka et al.
CBS	cystathionine beta-synthase, selanocystathionine beta-synthase	Methionine Metabolism	yes	Homozygous mice die within 5 weeks after birth	Watanabe et al.
SPTLC2	serine C-palmitoyltransferase	Sphingolipid Metabolism	yes	Embryonic lethality of homozygotes	Hojjati et al.
PAH	L-Phenylalanine,tetrahydrobiopterin:oxygen oxidoreductase	Tyr, Phe, Trp Biosynthesis	no	Homozygous mice with dysruptions in the gene are viable	http://www.informatics.jax.org
TM7SF2	C-14 sterol reductase	Cholesterol Metabolism	no	Although a mixture of mutations can be lethal, homozygotes for mutations are viable	http://www.informatics.jax.org
Gpam	glycerol-3-phosphate acyltransferase	Triacylglycerol Synthesis	no	Homozygous mice are viable	Howerton et al.