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MvaT oligomerization mutants are impaired for higher-order oligomer formation in vitro. In vitro cross-linking experiment of wild-type and mutant forms of MvaT (F36S and R41P). Glutaraldehyde (0.04%) was added to purified C-terminal His-tagged proteins (1.85 µM). Aliquots were removed at the indicated time points and proteins analyzed by Western blotting using an antibody against the His tag on each protein. Different oligomeric species are indicated.
