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Dominant-negative effect of MvaT(F36S) and MvaT(R41P). PAO1 cupA1 lacZ cells harboring either the empty control vector pPSV37 (indicated -), pPSV-MvaT-V (indicated MvaT), pPSV-MvaT(F36S)-V (indicated F36S), or pPSV-R41P-V (indicated R41P), were grown at 37°C to mid-logarithmic phase in the presence of 1 mM IPTG and assayed for β-galactosidase activity.
