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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 29;78(4):903–915. doi: 10.1111/j.1365-2958.2010.07376.x

Fig. 2.

Fig. 2

Hmp and NorV confer protection from NO inhibition on growth, depending on the oxygen availability. Growth in minimal-salts medium plus GlcNAc, was monitored at OD600. Aerobic cultures (A and B) were challenged with 100 μM of the NO-donor DEA-NONOate (thick arrow), when growth reached early log phase. To pre-adapt the cells (B), a pretreatment of 40 μM DEA-NONOate was added (thin arrow) 45 min before the challenge. Similarly, anaerobic cultures (C and D) were challenged with 40 μM DEA-NONOate (thick arrow). To pre-adapt the cells (D), 5 μM DEA-NONOate was added (thin arrow) 45 min before the challenge. Growth, either without (filled symbols) or with (open symbols) pretreatment, was monitored for the wild-type (circle), Δhmp (triangle), ΔnorV (dash line), Δhmp-norV (cross) and ΔnsrR (square) strains. Experiments were repeated three times with similar results. One representative experiment is shown here. Data points are the means calculated from the three technical replicates of that experiment.