Table 1.

NsrR is the major NO-responsive negative regulator of hmp.

Comparison	Relativehmpexpression*
	Aerobic	Anaerobic
ΔnsrR/WT†	231 ± 33	250 ± 44
ΔnsrR+NO/WT+NO‡	1.0 ± 0.2	1.6 ± 0.2
ΔnsrR+NO/ΔnsrR	0.9 ± 0.1	2.3 ± 0.3

^*The ratio of expression levels of hmp transcripts in each pair of strains being compared as measured by qRT-PCR.

^†WT; wild type

^‡Cultures were exposed to NO by adding either 80 μM (aerobic conditions) or 5 μM (anaerobic conditions) DEA-NONOate.