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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Nov;78(4):837–852. doi: 10.1111/j.1365-2958.2010.07304.x

Fig. 3. In vitro assay of H. pylori Kdo hydrolase mutants.

Fig. 3

Membranes from both Hp0580 and Hp0579-Hp0580 mutants in strains G27 and 7.13 were assayed using 1-dephosphorylated-Kdo2-[4′-32P]lipid A as the substrate. Each of the mutants showed a complete loss of Kdo hydrolase activity, which was fully restored in complemented strains.