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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Nov;78(4):837–852. doi: 10.1111/j.1365-2958.2010.07304.x

Fig. 6. Mass spectrometry of the G27 Kdo hydrolase mutant.

Fig. 6

Lipid A was isolated from G27, G27 hp0579-80::cam and G27 hp0579-80::cam, hp0579-80+ and analyzed by MALDI-TOF mass spectrometry in the negative-ion mode. G27 produced a peak at 1547.9 m/z corresponding to the published wild type H. pylori lipid A mass (A). In addition to the wild type peak at m/z 1547.7 G27 hp0579-80::cam also displayed a peak at m/z 2091.0 (B). 2091.0 corresponds to a hexa-acylated lipid A species, with the 4′-phosphate still present. G27 hp0579-80::cam, hp0579-80+ showed complete reversion to the wild type phenotype, with a single peak present at a m/z of 1547.4 (C).