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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Nov;78(4):837–852. doi: 10.1111/j.1365-2958.2010.07304.x

Fig. 7. SDS-PAGE analysis of Kdo hydrolase mutants and complements.

Fig. 7

Proteinase K treated whole cell lysates from Hp0580, Hp0579-Hp0580 mutants and Hp0580, Hp0579-Hp0580 complemented mutants, in strains G27 and 7.13, were separated by SDS-PAGE and stained with Pro-Q Emerald 300 Lipopolysaccharide Gel Stain Kit (A) or blotted to a nitrocellulose membrane and probed with antibodies raised against various human blood group antigens (B). The Kdo hydrolase mutants in both strains displayed a reduced amount of fully extended O-antigen and an increase in core-lipid A, as compared to wild type. A wild type phenotype was fully restored in each of the complemented Kdo hydrolase mutants. Strain 7.13 only expressed the H type 1 blood group antigen, which was reduced in the Kdo hydrolase mutants. Strain G27 expressed both Lewis X and Lewis Y blood group antigens. Lewis Y expression was unaffected in the Kdo hydrolase mutants; however, Lewis X expression was absent in each of the Kdo hydrolase mutants.