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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Gene Ther. 2010 Jul 22;17(12):1430–1441. doi: 10.1038/gt.2010.100

Figure 1. The Ad replication cycle further increases HIF transcriptional activity under hypoxia rather than inhibiting its activity.

Figure 1

LN229 (a) and U251MG-T2 (b) cells were transfected with the pBIGL-V6R plasmid which contains a luciferase reporter gene under the regulation of a HIF-responsive promoter. Cells were then infected with a wild-type Ad (dl309-Ad) at the indicated multiplicity of infection (MOI) and incubated under normoxia versus hypoxia. The next day, luciferase activity was measured and normalized to total cellular protein levels (light units/μg protein). The data represent the mean ± SD.