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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 23;78(4):866–882. doi: 10.1111/j.1365-2958.2010.07369.x

Figure 4.

Figure 4

YneA and Noc inhibit cell division when all replication is blocked by the inhibitor HPUra. Wild-type (BDR11), ΔyneA (BRB12), Δnoc (BRB73) and the double mutant (BRB89) were subjected to HPUra treatment and visualized by fluorescence microscopy. (A) Representative fields of cells 80 min after addition of HPUra. Membranes were stained with FM4-64 (red) and DNA was stained with DAPI (false-colored green). Septation events adjacent to or on top of the nucleoid are highlighted (yellow carets). White bar is 1 μm. (B) Average cell length measurements following HPUra treatment. Values are the average from 3 independent experiment (±SD), >1500 cells were measured for each strain and time point. (C) Histogram quantifying different septation events 80 min after addition of HPUra. Septations were binned into 4 classes described in the text. A representative picture of each class is shown below the x-axis. The fraction of each class was calculated relative to the total numbers of septation events monitored (n=500). Values and standard deviations are based on data from 3 independent experiments.