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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 23;78(4):866–882. doi: 10.1111/j.1365-2958.2010.07369.x

Figure 6.

Figure 6

Altering chromosome compaction and/or organization relieves cell division inhibition on top of the DNA mass during fork arrest. (A) Representative images obtained 45 min after induction of the replication roadblock in wild-type (strain BRB150), Δspo0J (strain BRB225), and during SMC depletion (strain BRB359). Membranes were stained with FM4-64 (red), DNA was stained with DAPI (blue) and TetR-GFP foci are shown in green. Septa that form on top of the DNA are highlighted (yellow carets). White bar is 1μM. (B) Histogram quantifying the different septation events in the indicated strains 45 min after induction of the replication roadblock. Septations were binned into 4 classes described in the text. A representative picture of each class is shown below the x-axis. The fraction of each class was calculated relative to the total numbers of septation events monitored (n=600). Average values and standard deviations are from 3 independent experiments.