Fig. 1.

The concentration dependence of IL-8 release by rGrA and thrombin in A549 cells. A549 cells were incubated for 24 h with SFM, SFM containing rGrA, and SFM containing thrombin. After incubation, concentration of IL-8 in the conditioned medium was determined using an ELISA kit. The concentrations for rGrA (from left to right) are 0, 100, 150, 200, 300, 600, and 900 nM. The concentrations for thrombin (from left to right) are 0, 1, 5, 15, 25, 50, and 100 nM. Data are expressed as means ± SE from three independent experiments performed in duplicate. ** and * P < 0.01 and P < 0.05, respectively, versus A549 cells incubated with SFM alone (two-tailed, unpaired t-test)