Fig. 1.

In vitro effects of Long-du extract (LDE) on the growth in B16M cells (a) and γ-radiation-treated B16M cells (b). a B16M cells were cultured for 24, 48, and 72 h in DMEM medium containing LDE at the concentrations indicated in figure. The effects of LDE on the cell growth were determined by MTT assay as described in the “Materials and methods” section. b B16M cells were treated with γ-radiation at dose of 8 Gy in the absence or presence of LDE at 0.4 mg/mL. After the treated cells were cultured for 48 h, the relative cell viability was determined using the trypan blue dye exclusion assay as described in the “Materials and methods” section. Each point and vertical bar represents the mean and SD for 6 wells. The figures (a, b) are the representative of three similar experiments performed. Statistical analysis was carried out using the ANOVA and Bonferroni test. All statistical tests were two-sided. Values with different letters (a–c) differ significantly (P < 0.05)