Figure 6.
Time course of in vitro translation of p94 and the μ-calpain large subunit (μCL). In vitro transcribed RNAfor rat p94 and human μCL were translated with rabbit reticulocyte lysate and [35S]Met in the presence (+inh.) or absence (−inh.) of 380 μg/mL leupeptin and E64, and sampled at the indicated time (min). Samples are separated by SDS-PAGE, and exposed to X-ray film (A) and an imaging plate. The radioactivities of 94-kDa (open triangle) and 80-kDa (closed triangle) bands were quantified using a Fujix Bioimage Analyzer, and plotted against the time of sampling (B). Note that the scales for the Y-axes differ by a factor of 10.