FIG. 1.

(a) Schematic diagram of the BAC construct used for generating rBDNF-lacZ-BAC transgenic mice (thick lines). White boxes represent untranslated sequences and the blue filled box represents lacZ reporter gene that replaces the BDNF coding sequence. rBDNF-CAT constructs (I–III and IV–VI) used by Timmusk et al. (1995) to generate rBDNF transgenic mice are shown with asterisks. (b) RT-PCR analysis of rBDNF-lacZ mRNA expression driven by rBDNF promoters in transgenic mouse tissues. Abbreviations: mBDNF, mouse BDNF; HPRT, hypoxanthine phosphoribosyltransferase 1; Cx, cortex; Hc, hippocampus; Cb, cerebellum; OB, olfactory bulb; TH, thalamus and hypothala-mus; PM, pons/medulla; Mb, midbrain; St, striatum; Ty, thymus; He, heart; Lu, lung; Li, liver; Ki, kidney; SM, skeletal muscle; Sp, spleen; Te, testis. [Color figure can be viewed in the online issue, which is available at http://www.interscience.wiley.com.]