Table 3.
Thermal denaturation analysis
| 5′ UTR | No salt | LiCl | NaCl | KCl |
|---|---|---|---|---|
| EBAG9 | ||||
| wt | n.a. | n.a. | 51.4 ± 1.1 | >90 |
| mut | 58.0 ± 1.3 | 72.7 ± 2.1 | 72.2 ± 0.9 | 71.2 ± 1.1 |
| FZD2 | ||||
| wt | 65.1 ± 1.8 | 77.0 ± 1.9 | 79.3 ± 1.7 | >90 |
| mut | 58.8 ± 2.3 | 70.5 ± 0.1 | 68.0 ± 1.5 | 64.0 ± 0.1 |
| BARHL1 | ||||
| wt | 40.1 ± 1.0 | 44.2 ± 1.9 | 70.6 ± 3.1 | >90 |
| mut | 48.2 ± 0.6 | 64.7 ± 1.7 | 61.8 ± 2.7 | 61.2 ± 1.6 |
| NCAM2 | ||||
| wt | 67.6 ± 4.0 | 78.4 ± 1.4 | > 90 | > 90 |
| mut | 68.9 ± 1.7 | 70.4 ± 1.4 | 73.8 ± 2.8 | 72.1 ± 0.2 |
| THRA | ||||
| wt | 67.8 ± 1.4 | 76.0 ± 0.8 | 75.4 ± 1.1 | >90 |
| mut | 82.3 ± 1.1 | 82.4 ± 1.2 | 82.6 ± 2.2 | 81.5 ± 1.0 |
| AASDHPPT | ||||
| wt | 65.5 ± 1.8 | 77.7 ± 0.6 | 75.1 ± 4.7 | >90 |
| mut | 52.4 ± 0.7 | 59.2 ± 1.3 | 61.5 ± 0.2 | 59.1 ± 0.5 |
| snp | 59.9 ± 2.2 | 75.5 ± 3.7 | 75.2 ± 1.7 | 73.7 ± 0.9 |
| TNFSF12 C/A | ||||
| wt | 46.4 ± 3.1 | 60.5 ± 0.2 | 56.9 ± 0.9 | 79.8 ± 0.6 |
| muta | 49.0 ± 1.2 | 59.4 ± 1.9 | 62.5 ± 4.2 | 57.8 ± 1.9 |
| DOC2B C/A | ||||
| wt | 59.8 ± 2.0 | 65.6 ± 0.5 | 68.4 ± 0.1 | 74.1 ± 0.5 |
| mutb | 56.9 ± 0.4 | 64.3 ± 1.9 | 64.5 ± 0.1 | 63.3 ± 2.6 |
| MAP3K11 C/A | ||||
| wt | 59.1 ± 0.5 | 68.1 ± 1.7 | 67.8 ± 0.4 | 73.9 ± 0.3 |
| mutc | 54.0 ± 0.7 | 63.6 ± 0.1 | 64.1 ± 1.4 | 59.4 ± 2.0 |
n.a. The magnitude of the curves did not permit the determination of accurate Tm values.
aCorresponds to the TNFSF12 CG/AA-mutant version.
bCorresponds to the DOC2B CG/AA-mutant version.
cCorresponds to the MAP3K11 CG/AA-mutant version.