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. 2010 Jun 22;38(20):6906–6919. doi: 10.1093/nar/gkq559

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Brm is not expressed in erythroid cells of the fetal liver. (A) RT-qPCR analysis of Brg1 and Brm mRNA levels normalized to Gapdh levels in wild-type flow-sorted erythroid cells (TER119+) and whole mouse embryos minus their fetal livers (embryo) at E12.5. The white histograms on the left show Brg1 expression of embryos relative to TER119+ samples. The gray asterisk on the right indicates Brm mRNA levels are below the limit of detection in TER119+ samples and is compared to Brm expression in embryoss (gray histogram). Each histogram represents the mean ± SE for two (TER119+) or three (embryo) independent experiments. (B) Brm mRNA levels relative to Brg1 mRNA levels based on data presented in (A). For TER119+ (left) and embryo samples (right), Brg1 mRNA levels (white histograms) are set at 1.0 and Brm mRNA levels (gray asterisk/histogram) are shown in proportion. The asterisk indicates Brm was analyzed for TER119 samples but mRNA was not detected.