Table 2.
Putative R-M systemsa showing the highest sequence similarity to R.BspRI
| Organism | Putative REase |
Putative MTase |
Frequency of GGCC sitesb | ||||
|---|---|---|---|---|---|---|---|
| Protein | Length (aa) | E-value to R.BspRI | Protein | Length (aa) | E-value to M.BspRI | ||
| Acinetobacter haemolyticus ATCC 19194 | Conserved hypothetical protein ZP_06729282 | 302 | 5e−51 | ZP_06729281 (M.AhaBGORF3490P) | 336 | 3e−53 | 2/1900 |
| Gardnerella vaginalis ATCC 14019 | Conserved hypothetical protein ZP_03936874 | 298 | 3e−44 | ZP_03936873 (M.GvaORF417P) | 333 | 1e−52 | 1/1950 |
| Roseburia intestinalis L1-82 | Hypothetical protein RintL_00030 ZP_04741872 | 293 | 6e−41 | ZP_04741871 (M.RinLORF5004P) | 432 | 3e−149 | 0/2191 |
| Bacteroides sp. 3_1_33FAA | Conserved hypothetical protein ZP_06087297 | 297 | 7e−40 | ZP_06087299 (M.BspFAAORF965P) | 466 | 2e−49 | 0/2585 |
| Bacteroides ovatus SD CMC 3f | Conserved hypothetical protein ZP_06618190c | 298 | 3e−36 | ZP_06618187(M1.BovSDORF2192P) | 459 | 8e−51 | 3/4846 |
| ZP_06618188 (M2.BovSDORF2192P) | 337 | 8e−51 | |||||
| Lysinibacillus sphaericus C3-41 | Hypothetical protein Bsph_0498 YP_001696253 | 249 | 7e−32 | YP_001696252 (M.LspCORF497P) | 426 | 2e−148 | 1/2132 |
| Providencia alcalifaciens DSM 30120 | Hypothetical protein PROVALCAL_01484 ZP_03318550 | 295 | 7e−30 | ZP_03318551 (M.PalDORF1485P) | 330 | 2e−52 | 2/1927 |
| Uncultured marine crenarchaeote HF4000_APKG3B16 | Hypothetical protein ALOHA_HF4000APKG3B16ctg1g5 ABZ08412 | 300 | 2e−26 | ABZ08413 (M.UcrHFORF6P) | 377 | 2e−48 | 1/2026 |
| Streptococcus thermophilus CNRZ1066 | Hypothetical protein str0690 YP_141100d | 188 | 9e−15 | None | |||
MTase names (in parentheses) are from REBASE (1).
aIdentified by BLAST (blastp) search of the GenBank non-redundant protein database.
bNumber of BspRI recognition sites in the DNA regions (in base pairs) encompassing the ORFs of the predicted REases and the counterpart C5-MTases.
cOne of the flanking genes encodes a putative Vsr DNA mismatch endonuclease.
dOne of the flanking genes encodes a putative regulatory protein of an R-M system.