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mRev-erb α protein is not degraded by rapamycin treatment. The levels of mRev-erb α protein were analyzed by western blotting. mRev-erb α protein was rapidly decreased following cycloheximide treatment, but was insensitive to rapamycin. Phospho-rpS6 is a phosphorylated S6 ribosomal protein. 14-3-3 was used as a loading control. DMSO, dimethyl sulfoxide; CHX, cycloheximide.
