Table 2.
HD-deregulated miRNAs
| MiRNA | Expression pattern in HD (sequencing data) | Expression pattern in HD (miRNA arrays) | Expression pattern in HD (miRNA qRT-PCR) |
|---|---|---|---|
| hsa-let-7g | UP | UP | |
| hsa-miR-100 | UP | UP | UP |
| hsa-miR-101 | UP | – | |
| hsa-miR-106b | UP | UP | |
| hsa-miR-1250 | UP | NA | |
| hsa-miR-126 | UP | – | |
| hsa-miR-126* | UP | DOWN | |
| hsa-miR-1308 | UP | NA | |
| hsa-miR-143 | UP | DOWN | |
| hsa-miR-145 | UP | – | |
| hsa-miR-146a | UP | – | |
| hsa-miR-148b | UP | UP | |
| hsa-miR-151-3p | UP | UP | UP |
| hsa-miR-151-5p | UP | UP | |
| hsa-miR-15a | UP | UP | |
| hsa-miR-15b | UP | UP | |
| hsa-miR-16 | UP | UP | UP |
| hsa-miR-17 | UP | UP | |
| hsa-miR-181a | UP | UP | |
| hsa-miR-181a* | UP | UP | |
| hsa-miR-193b | UP | UP | |
| hsa-miR-197 | UP | DOWN | |
| hsa-miR-1974 | UP | NA | |
| hsa-miR-199b-3p | UP | UP | |
| hsa-miR-19b | UP | UP | |
| hsa-miR-204 | UP | UP | |
| hsa-miR-20a | UP | UP | |
| hsa-miR-219-2-3p | UP | UP | UP |
| hsa-miR-219-5p | UP | UP | |
| hsa-miR-22 | UP | DOWN | |
| hsa-miR-23a | UP | – | |
| hsa-miR-27b | UP | UP | UP |
| hsa-miR-29c* | UP | DOWN | |
| hsa-miR-30a | UP | DOWN | |
| hsa-miR-30b | UP | – | |
| hsa-miR-30c | UP | UP | |
| hsa-miR-30e | UP | – | |
| hsa-miR-338-3p | UP | UP | |
| hsa-miR-33a | UP | UP | |
| hsa-miR-33b | UP | UP | |
| hsa-miR-363 | UP | UP | |
| hsa-miR-374a* | UP | – | |
| hsa-miR-451 | UP | UP | UP |
| hsa-miR-484 | UP | DOWN | |
| hsa-miR-486-5p | UP | UP | |
| hsa-miR-574-3p | UP | DOWN | |
| hsa-miR-664 | UP | NA | |
| hsa-miR-887 | UP | UP | |
| hsa-miR-92a | UP | UP | UP |
| hsa-miR-93 | UP | UP | |
| hsa-miR-99b | UP | UP | |
| hsa-let-7a | DOWN | – | |
| hsa-let-7c | DOWN | – | |
| hsa-let-7d | DOWN | – | |
| hsa-let-7e | DOWN | – | |
| hsa-miR-103 | DOWN | DOWN | |
| hsa-miR-107 | DOWN | DOWN | |
| hsa-miR-1224-5p | DOWN | NA | |
| hsa-miR-124 | DOWN | DOWN | |
| hsa-miR-127-3p | DOWN | DOWN | |
| hsa-miR-128 | DOWN | DOWN | DOWN |
| hsa-miR-1301 | DOWN | NA | |
| hsa-miR-1307 | DOWN | NA | |
| hsa-miR-139-3p | DOWN | DOWN | DOWN |
| hsa-miR-181d | DOWN | DOWN | |
| hsa-miR-193b* | DOWN | – | |
| hsa-miR-199a-3p | DOWN | NA | |
| hsa-miR-221 | DOWN | DOWN | |
| hsa-miR-222 | DOWN | DOWN | DOWN |
| hsa-miR-323-3p | DOWN | DOWN | |
| hsa-miR-330-3p | DOWN | UP | |
| hsa-miR-369-5p | DOWN | DOWN | |
| hsa-miR-382 | DOWN | DOWN | DOWN |
| hsa-miR-383 | DOWN | DOWN | – |
| hsa-miR-409-5p | DOWN | DOWN | |
| hsa-miR-423-5p | DOWN | – | |
| hsa-miR-432 | DOWN | DOWN | |
| hsa-miR-433 | DOWN | DOWN | DOWN |
| hsa-miR-485-3p | DOWN | DOWN | DOWN |
| hsa-miR-485-5p | DOWN | DOWN | |
| hsa-miR-92b* | DOWN | – | |
| hsa-miR-495 | DOWN | DOWN | |
| hsa-miR-543 | DOWN | DOWN | |
| hsa-miR-598 | DOWN | DOWN | |
| hsa-miR-708 | DOWN | – | |
| hsa-miR-760 | DOWN | UP | |
| hsa-miR-95 | DOWN | DOWN |
The differential expression in the sequenced samples considers miRNAs commonly deregulated in HD-FC and HD-ST >1.2 and <–1.2.
P < 0.05 (Z-test, Hochberg and Benjamini correction).
The reference miRNA and all the variants mapping on the same miRNA locus are considered in this analysis.
Only sequences with a normalized frequecy cutoff above 50 (norm freq C-FC + norm freq HD-FC > 50) and (norm freq C-ST + norm freq HD-ST >50) were included.
In the miRNA arrays, a fold change above 1.2 and below –1.2 in absolute value was considered.
The results showing a false discovery rate-adjusted P-value (FDR) below % are highlighted in black, and those with a P > 10% are shown in light gray.
Selected miRNAs (seven uregulated and seven downregulated) were chosen for validation by RT-PCR.
The signifficantly deregulated miRNAs (P < 0.01) are highlighted in black.
(−) Means that no deregulation was found.
(NA) Means that the miRNA was not available in the array.
In bold, the miRNAs with a more consistent deregulation pattern using different techniques in additional samples are shown.