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. 2010 Jul 6;38(20):6976–6984. doi: 10.1093/nar/gkq576

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — (A) Chemical structures of MC, MC-mono-dG, and ICL dG-MC-dG DNA adducts. (B) The DNA sequence of the 61-bp DNA fragment containing a site-specific MC-mono-dG (substrate 1) or an ICL dG-MC-dG adduct (substrate 2). G* represents MC-modified deoxyguanosine. Restriction enzyme SmaI site is indicated. (C) Substrate 1, substrate 2 and control 61-bp DNA fragments were 5′-end ³²P labeled, denatured and then separated by electrophoresis in a denaturing gel.