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. 2010 Jul 2;38(20):7179–7186. doi: 10.1093/nar/gkq584

Figure 3.

Figure 3.

Proton release and hydrolysis events limit the observed steady-state rates in the hDUTF158A mutant. (A) Time courses of proton release upon mixing 15 µM hDUTF158A with buffer or with sub-stoichiometric (10 µM) dUTP in the stopped-flow. Single exponential fit to the curve yielded kobs = 0.23 ± 0.007 s−1. (B) Time courses of STO quench-flow experiments upon mixing hDUT or hDUTF158A with sub-stoichiometric radioactive dUTP. The inset shows the same on a logarithmic time scale. Calculated rate constants are summarized in Table 1.