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. 2010 Jul 12;38(20):7133–7141. doi: 10.1093/nar/gkq610

Figure 2.

Figure 2.

Fosmids containing inserts of S. enterica serovar Cerro 87 DNA express a restriction phenotype in E. coli DH5α. (A) dptB-E region of S. enterica serovar Cerro 87 with the probe DNA that was used to identify six fosmid clones shown below at a reduced scale. The fosmids were aligned relative to each other according to their EcoRI (and other) restriction patterns. (B) Genomic DNA of E. coli DH5α containing the six S. enterica serovar Cerro 87 fosmids fractionated using PFGE. Extensive degradation occurred in all the DNA samples except in the sulfur-free sample from E. coli DH5α. (C) Restriction of S-free and S-modified pKOV-Kan in different E. coli DH5α containing the six S. enterica serovar Cerro 87 fosmids. The results were presented as relative transformation efficiencies (ratios of S−/S+ plasmid) obtained by parallel transformation of S− and S+ plasmid DNA. Error bars represent the standard deviation from three repeat experiments. S. 87, S. enterica serovar Cerro 87; XTG102, S. enterica serovar Cerro 87 mutant strain with deletion of dptBCDE.