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. 2010 Jul 12;38(20):7133–7141. doi: 10.1093/nar/gkq610

Figure 3.

Figure 3.

Mutational analysis of the dpt gene cluster of S. enterica serovar Cerro 87. (A) Mutational analysis of the dpt gene cluster. The complete, functional dptB-H cluster (including ORF5) was cloned in pJTU3818 and expressed in E. coli DH5α. Mutations were created in plasmids and in the S. enterica serovar Cerro 87 genome (XTG strains). White boxes with gene designations indicate deleted regions. (B) Restriction of S-free and S-modified pKOV-Kan in different mutantion strains. The results were presented as relative transformation efficiencies (ratios of S−/S+ plasmid) obtained by parallel transformation of S− and S+ plasmid DNA. Error bars represent the standard deviation from three repeat experiments. S. 87, S. enterica serovar Cerro 87; XTG102, S. enterica serovar Cerro 87 mutant strain with deletion of dptBCDE; XTG105, S. enterica serovar Cerro 87 mutant strain with in-frame deletion of dptG; XTG104, S. enterica serovar Cerro 87 mutant strain with in-frame deletion of dptF.