Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Salmonella enterica | ||
| 87 | Serotype Cerro, Dnd+ | (4) |
| XTG101 | 87 derivative, dptC interruption mutant | This work |
| XTG102 | 87 derivative, dptB-E deletion mutant | This work |
| XTG104 | 87 derivative, dptF in-frame deletion mutant | This work |
| XTG105 | 87 derivative, dptG in-frame deletion mutant | This work |
| Escherichia coli | ||
| DH5α | F- recA lacZ ΔM15 | (17) |
| EPI300TM-T1R | used for construction of genomic library | EPICENTER |
| B7A | Dnd+ | |
| Plasmids | ||
| pBluescript II SK(+) | Cloning vector, Ampr | (18) |
| pMD18-T | vector for DNA sequencing | TaKaRa |
| pUC18 | cloning vector, Ampr | (19) |
| pKD46 | temperature-sensitive replication, Ampr | (20) |
| pKOV-Kan | temperature-sensitive replication, sacB, Cmr, Kanr | (21) |
| pCC1FOSTM | Cmr oriV ori2 | EPICENTER |
| 8F4 | pCC1FOSTM derived fosmid carrying dpt cluster | This work |
| 6G12 | pCC1FOSTM derived fosmid carrying dpt cluster | This work |
| pJTU1231 | pMD-18T derivative carrying a 0.9-kb PCR product of p10/p11 | This work |
| pJTU1232 | pUC18 derivative carrying a 4.4-kb EcoRI fragment from 8F4 | This work |
| pJTU1233 | pUC18 derivative carrying a 3.4-kb EcoRI fragment from 8F4 | This work |
| pJTU1237 | pBluescript II SK(+) derivative carrying a 2.5-kb EcoRI–ScaI/EcoRV fragment from pJTU1233 | This work |
| pJTU1238 | pJTU1237 derivative carrying 6.7-kb dptB-E region | This work |
| pJTU1239 | pKD46 derivative carrying a 0.7-kb EcoRI–EcoRV fragment of dptC from pJTU1231 | This work |
| pJTU2489 | pUC18 derivative carrying a 5.9-kb PvuII-SmaI fragment from 6G12 | This work |
| pJTU2492 | pJTU2489 derivative carrying a 8.9-kb NsiI fragment from 6G12 | This work |
| pJTU3809 | pJTU2492 derivative with 4.7-kb SalI region deletion | This work |
| pJTU3818 | pCC1FOSTM derivative carrying a 20-kb BglII fragment from 6G12 | This work |
| pJTU2468 | pKOV-Kan derivative carrying a 1.5-kb BamHI/XhoI digested PCR product with deletion in dptB-E | This work |
| pJTU3825 | pUC18 derivative carrying a 1.1-kb PCR product of FLL/FRR for sequencing | This work |
| pJTU3826 | pUC18 derivative carrying a 1.1-kb PCR product of GLL/GRR for sequencing | This work |
| pJTU3828 | pKOV-Kan derivative carrying 1.1-kb BamHI-SalI region from pJTU3825 with in-frame deletion in dptF | This work |
| pJTU3829 | pKOV-Kan derivative carrying 1.1-kb BamHI-SalI region from pJTU3826 with in-frame deletion in dptG | This work |
oriT, origin of transfer of plasmid RK2; Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Kanr, Kanamycin resistance; sacB, levansucrase gene of Bacillus amyloliquefaciens.