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. 2010 Jul 7;38(20):7260–7272. doi: 10.1093/nar/gkq611

Figure 4.

Figure 4.

The p17 translation start site obstructs downstream translation initiation. (A) The parental σC-RL transcript was modified by progressive 5′-truncations (Δ19, Δ27, Δ79, Δ147, Δ303; numbers refer to the number of 5′-terminal nucleotides deleted from the σC-RL mRNA sequence) (top panel). Luciferase activity (bottom panel) of the parental construct (σC-RL) and the 5′-truncations were determined as described in Figure 2C. (B) The σC-RL construct was modified to eliminate the four AUG start codons (M1–M4; AUG to AUC) that reside upstream of the σC start codon, either individually or in various combinations (top panel). Luciferase activity (bottom panel) of the parental (σC-RL) and modified constructs were determined as described in Figure 2C. All results are reported as mean ± SE (n = 3) of the relative level of σC-RL translation, normalized to the parental σC-RL construct. Expression levels significantly different from the parental construct (***P < 0.001 and **P < 0.01) are indicated.