Figure 2.

Methylation dependence of proteins identified by a single-bait one-hybrid screening. (A) Schematic representation of the single-bait/dual-reporter system. The results of the screening using this system are summarized in Table 1. X, methylated DNA-binding protein; BTA, basic transcription apparatus. Filled circle shown on the bait, methylated CpG. (B) Methylation dependence of DNA-binding proteins identified by the single-bait one-hybrid screening. One-hybrid interaction with the Sm₄ bait was examined for each protein using a lacZ reporter plasmid pOp₈Sm₄ (7) in the absence and presence of pALMS that expresses LexA-M.SssI. β-galactosidase activity in the extract of each transformant was measured using a standard method (9).