FIG. 3.
Packaging cell line-derived HMGB1 protein is associated with purified HSV amplicon viral particles. A 10-μl aliquot (equivalent to ∼5 × 106 transduction units) of each HSV-1 amplicon vector packaged from the BHK cell line was lysed in RIPA buffer and subjected to Western blot analysis to determine the presence of HMGB1 protein derived from the BHK cell line (top). On HMGB1 immunoblotting, the blots were stripped and subsequently analyzed for the presence of the HSV tegument protein VP16 (bottom). Molecular masses are represented as kilodaltons (kDa).