FIG. 3.

Induction of hiPSCs from HFFs with the FRh11 encoding human reprogramming factors. (A) Time schedule used for reprogramming of HFFs. (B) Comparison of expression levels of reprogramming factors in 293T cells infected with the MLV vector (pMXs) or the lentivirus vector (FRh11). 293T cells were infected with either pMXs or FRh11 encoding each human reprogramming factor at an MOI of 1. The cells were lysed with 0.5% SDS 48 hr post infection and analyzed by western blotting for Oct4, Sox2, Klf4, and cMyc expression. Non, noninfected 293T cells; NTERA-2, human EC cells. (C) Morphological changes of representative colonies on days 7, 14, and 21 after infection of lentiviral vectors encoding each reprogramming factor. (D) Representative image of AP-positive colonies on day 28 after infection. Color images available online at www.liebertonline.com/hum.