Table 1.
Primers for RT-PCR
| Genes | Forward (5′ to 3′) | Reverse (5′ to 3′) | Size (bp) |
|---|---|---|---|
| Endogenous | |||
| NANOG | CAGCCCTGATTCTTCCACCAGTCCC | GGAAGGTTCCCAGTCGGGTTCACC | 390 |
| REX1 | CAGATCCTAAACAGCTCGCAGAAT | GCGTACGCAAATTAAAGTCCAGA | 305 |
| LIN28 | ATCTGTAAGTGGTTCAACGTGCGC | TGGCAGCTTGCATTCCTTGGCATG | 338 |
| UTF1 | CCGTCGCTGAACACCGCCCTGCTG | CGCGCTGCCCAGAATGAAGCCCAC | 147 |
| DPPA5 | ATATCCCGCCGTGGGTGAAAGTTC | ACTCAGCCATGGACTGGAGCATCC | 242 |
| TERT | TGTGCACCAACATCTACAAG | GCGTTCTTGGCTTTCAGGAT | 165 |
| DNMT3B | ATAAGTCGAAGGTGCGTCGT | GGCAACATCTGAAGCCATTT | 121 |
| OCT4 | CCTCACTTCACTGCACTGTA | CAGGTTTTCTTTCCCTAGCT | 163 |
| SOX2 | CCCAGCAGACTTCACATGT | CCTCCCATTTCCCTCGTTTT | 150 |
| cMYC | TGCCTCAAATTGGACTTTGG | GATTGAAATTCTGTGTAACTGC | 191 |
| KLF4 | GATGAACTGACCAGGCACTA | GTGGGTCATATCCACTGTCT | 144 |
| GAPDH | GAAGGTGAAGGTCGGAGT | GAAGATGGTGATGGGATTTCC | 225 |
| Total | |||
| OCT4 | AGCGAACCAGTATCGAGAAC | TTACAGAACCACACTCGGAC | 140 |
| SOX2 | AGCTACAGCATGATGCAGGA | GGTCATGGAGTTGTACTGCA | 125 |
| cMYC | ACTCTGAGGAGGAACAAGAA | TGGAGACGTGGCACCTCTT | 158 |
| KLF4 | TCTCAAGGCACACCTGCGAA | TAGTGCCTGGTCAGTTCATC | 104 |
| Vector | |||
| OCT4 | GCTCTCCCATGCATTCAAACTGAGG | GGAGCAACATAGTTAAGAATACCAGTC | 167 |
| SOX2 | GACTTCACATGTCCCAGCACTACC | GGAGCAACATAGTTAAGAATACCAGTC | 361 |
| cMYC | GAACAGCTACGGAACTCTTGTGCG | GGAGCAACATAGTTAAGAATACCAGTC | 203 |
| KLF4 | AGCATTTTCCAGGTCGGACCACC | GGAGCAACATAGTTAAGAATACCAGTC | 328 |