Table 2.
Comparing Derivation Efficiencies of hiPSC Colonies by Ectopic Expression of Reprogramming Factors
| Trial | Reprogramming factors | Therapeutic gene | No. of iPSC colonies | No. of established colonies | Derivation efficiency (%) |
|---|---|---|---|---|---|
| 1 | Oct4+Sox2+Klf4+cMyc | None | 22 | 20 | 0.04 |
| 2 | Oct4+Sox2+Klf4+cMyc+Nanog+Lin28 | None | 20 | 17 | 0.034 |
| 3 | Oct4+Sox2+Klf4+cMyc | sh1005a | 6b | 3 | 0.016 |
| 4 | Oct4+Sox2+Klf4+cMyc | mutant sh1005a | 7b | 5 | 0.024 |
| Total | 55 | 45 | 0.029c |
a
Short hairpin RNA (shRNA) directed to human chemokine receptor CCR5, designated as sh1005, and the 3-nt mismatch mutant (mutant sh1005) (Shimizu et al., 2009b).
b
Only colonies expressing EGFP were picked and counted.
c
The average of hiPSC derivation efficiency from four independent trials.