FIG. 5.

Microglial/macrophage staining pattern in mouse brain on P21 after in utero HSV/SB vector transduction. Immunostaining of brain-resident microglia/macrophage was performed according to an Iba-1-specific diaminobenzidine (DAB) protocol. A rostral-to-caudal display of representative P21 mouse brain sections corresponding to each of the treatment groups [HSV-HSB5 plus HSVT-βgeo (A–C), HSV-SB10 plus HSVT-βgeo (D–F), and HSVPrPUC plus HSVT-βgeo (G–I)] is presented. Photomicrographs (A), (D), and (G) correspond to the M1 region of the motor cortex; (B), (E), and (H) correspond to the CA1 region of the hippocampus (about –1.58 mm bregma); and (C), (F), and (I) correspond to the dentate gyrus region (about –2.46 mm bregma). Photomicrographs were obtained at an original magnification of × 10. Scale bar (A): 500 μm. Each inset [in (C), (F), and (I)] represents a digitally magnified ( × 20) image of the photomicrograph for better visualization of stained cell morphology. The number of Iba-1-positive microglia was counted within the visual motor (VM) cortex and hippocampal region of the brain, using an Olympus AX-70 microscope equipped with a motorized stage and MCID 6.0 Elite software (J). Six equivalent sections of the VM cortex and hippocampus region were separately counted and averaged per mouse at a magnification of × 20 (n = 3 mice per group). Error bars represent the standard error of the mean and statistical analysis was conducted by Student t test.