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Point mutations of maspin. a, ribbon diagram of maspin showing the positions of the mutations generated (PDB entry 1XU8, Chain B (15)). b, Western blots of maspin protein expression in DU145 cell lysate and conditioned media after transient transfection, detected with 2 μg/ml anti-maspin. Media samples were concentrated 20-fold by trichloroacetic acid precipitation. No additional bands were detected on the blot.
