FIGURE 6.

The furin inhibitor Dec-RVKR-CMK blocks processing of FGF23 and ANGPTL3 reporter constructs in CHO ldlD cells. Shown is anti-EYFP SDS-PAGE Western blot analysis of culture medium of cells grown with inhibitor as indicated. Cells were grown in Gal alone without capacity for O-glycosylation. Stable CHO ldlD clones were grown to 50% confluence in 6-well dishes, and the medium was replaced with medium containing a 100, 50, 25, 10, or 0 μm concentration of the cell-permeable Furin-inhibitor Dec-RVKR-CMK in DMSO. After 24 and 48 h of growth, cell culture supernatant was sampled. The arrows indicate the non-processed reporter and the cleaved N-terminal fraction that migrates at ∼48 and 39 kDa, respectively.