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Furin time course digestion of ANGPTL3 and FGF23 peptides monitored by MALDI-TOF analysis. Furin cleavage reactions were sampled at 0.5, 1.5, and 5 h. The dodecapeptide ANGPTL3 was cleaved in the RAPR↓ motif after 5 h, yielding an N-terminal fragment of 724 Da as expected. The FGF23 peptide was readily cleaved in the RHTR↓ motif, yielding the N-terminal fragment of 1032 Da after 30 min.
