TABLE 1.
Proteins involved in HDL and/or TG metabolism with established or predicted O-glycosylation sites
| Gene | Ensembl | ORF | Potential O-glycosylation sitesa | Established O-glycosylation sites | Target sequenceb | T1 | T2 | T3 |
|---|---|---|---|---|---|---|---|---|
| ABCA1 | ENSP00000363868 | 2261 | 0 | NDc | 251SKELAEATKTLLHSLG267 | NAd | NA | NA |
| APOA1 | ENSP00000364478 | 245 | 2 | ND | 99YPPTSMSRSPPTVLVI115 | NA | NA | NA |
| APOA2 | ENSP00000356969 | 61 | 1 | Yese | 46NFLSYFVELGTQPATQ61 | 1/2f | 0/2 | 0/2 |
| ANGPTL3 | ENSP00000360170 | 460 | 1 | ND | 216LSSKPRAPRTTPFLQL231 | 0/4 | 1/4 | 1/4 |
| BMP-1 | ENSP00000305714 | 986 | 0 | ND | 748DHKVTSTSGTITSPNW763 | NA | NA | NA |
| CETP | ENSP00000200676 | 493 | 1 | ND | 261EDLPLPTFSPTLLGDS277 | NA | NA | NA |
| LCAT | ENSP00000264005 | 440 | 3 | Yese | 424QGPPASPTASPEPPPP439 | 3/3 | 1/3 | 2/3 |
| LDLR | ENSP00000252444 | 860 | 6 | Yesg | 734TAVRTQHTTTRPVPDT749 | NA | NA | NA |
| LIPG | ENSP00000261292 | 500 | 1 | ND | 485DGWRMKNETSPTVELP500 | NA | NA | NA |
| PLTP | ENSP00000335290 | 441 | 10 | ND | 426RPADVRASTAPTPSTA439 | 3/5 | 2/5 | 3/5 |
| VLDLR | ENSP00000371532 | 873 | 2 | Yesg | 777PGGINVTTAVSEVSVP792 | NA | NA | NA |
| VNN-1 | ENSP00000356905 | 513 | 1 | ND | 458GRLFSLKPTSGPVLTV473 | NA | NA | NA |
| SCARB1 | ENSP00000261693 | 509 | 1 | ND | 489AYSESLMTSAPKGSVL504 | NA | NA | NA |
a Sites predicted by NetOGlyc 3.1 (available on the World Wide Web).
b Peptide sequence containing all or some of the potential O-glycosylation sites. Ser and Thr potential O-glycosylation sites are underlined. Sites predicted by NetOGlyc are shown in boldface type, and experimentally verified sites are shown in underlined boldface type.
c ND, not determined.
d NA, not analyzed.
e O-glycosylation-identified, determined sites in underlined boldface type.
f Sites incorporated in vitro by GalNAc-transferases in this study (number of GalNAc/total Ser/Thr sites).
g O-glycosylation identified, but sites not determined.