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. 2010 Sep 14;285(47):36330–36338. doi: 10.1074/jbc.M110.136465

FIGURE 2.

FIGURE 2.

Effects of various agents on ERK and Ser23 α1 subunit phosphorylation in parotid acinar cells. A, Western blots of cells treated as described in B. Cell lysates were probed as indicated. Ouab, ouabain; CCh, carbachol; Iono, ionomycin; Nys, nystatin; I, isoproterenol; F, forskolin. P-S23-α1, α1 subunit Ser23 phosphorylation; P-ERK, ERK phosphorylation. B, the phosphorylation of the α1 subunit on Ser23 (S23-Alpha1) was quantified relative to basal conditions. Cells were treated with the following agents: ouabain (Oua, 1 mm, 3 min; n = 5), carbachol (10−5 m, 2 min; n = 12), ionomycin (10−6 m, 2 min; n = 3), nystatin (10−4 m, 2 min; n = 3), PMA (100 nm, 2 min; n = 10), isoproterenol (Iso, 10−5 m, 2 min; n = 6), forskolin (Fsk, 10 μm, 2–12 min; n = 6), calyculin A (CA, 100 nm, 10 min; n = 4). *, p < 0.01 and **, p < 0.05 versus basal. C, Western blot of cell lysates and α1 subunit immunoprecipitates (IP: α1, 4 h). Cells were treated as described in B, except for calyculin A (Caly, 100 nm, 5 min).