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. 2010 Sep 17;285(47):36401–36409. doi: 10.1074/jbc.M110.172866

FIGURE 4.

FIGURE 4.

E4BP4 mediates feeding-induced FGF21 gene suppression. A, the mRNA levels of gluconeogenic genes, including G6pase, Pepck, and Pgc1α, in the fasted or fed liver tissues isolated from wild-type C57BL6 mice (n = 5). Data are presented as mean ± S.E. (error bars). *, p < 0.05. B, Q-PCR analysis for the mRNA level of E4bp4 in the same set of liver tissues (n = 5). The expression levels of Fgf21 and PPARα were measured as well. Data are presented as mean ± S.E. *, p < 0.05. C, immunoblotting analysis for the liver E4BP4 protein level from the same tissue samples. D, Q-PCR analysis of the E4bp4 mRNA levels in both the liver and adipose tissues isolated from fasted or fed wild-type C57BL6 mice (n = 5). Data are presented as mean ± S.E. *, p < 0.05. E, ChIP assay for the occupancy of E4BP4 on the Fgf21 promoter in both fasting and feeding conditions. Liver tissues (n = 3) harvested after fasting or feeding for 24 h were used as input materials. Data are presented as mean ± S.E. *, p < 0.05.