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SPR analysis of the ERj1-ribosome and ERj1-BiP interaction. GST-ERj1 (A and B) or GST-ERj1-H89Q (C and D) was immobilized on an activated CM5 sensor chip in the measuring cell. A and C, increasing concentrations of canine pancreatic ribosomes were passed over the chip, followed by buffer application. B and D, increasing concentrations of BiP or 10 μm BiP, respectively, was passed over the chip, followed by buffer application.
