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SPR analysis of the ERj1-ribosome interaction in the presence of BiP. GST-ERj1 (A and B) or GST-ERj1-H89Q (C and D) was immobilized on an activated CM5 sensor chip in the measuring cell. Where indicated, the chip was saturated with BiP. Increasing concentrations of canine pancreatic ribosomes were then passed over the chip in the presence or absence of BiP, followed by buffer application. Note that the chips were not regenerated after each ribosome injection (titration experiment).
